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Protocolos
Compounds › Hormonal and sexual

PT-141 (Bremelanotide)

MC3R/MC4R agonist — approved in the US as Vyleesi

Partially approvedHormonal and sexual

Experimental material. Read before using anything from here.

Nothing on this page is a medical recommendation, prescription or treatment plan. It is an organized translation of protocols circulating in research communities and, where it exists, of what published trials tested. The two are marked differently — and they are not equivalent.

Most of the compounds here have no FDA approval for human use. Several are sold labeled "research use only", which means they have not gone through purity, sterility or dosage controls for human consumption. A community-reported dose is not a validated dose: it is what someone reported having done.

Talk to a licensed health professional before considering any of these compounds. If you already use one and feel anything unexpected, seek care — do not wait for the next routine test.

Summary

Synthetic cyclic peptide that activates two melanocortin receptors in the brain, MC3R and MC4R. It is the active ingredient of Vyleesi, an FDA-approved injection for hypoactive sexual desire in premenopausal women. Outside that indication, use is off-label and the schedules come from the community.

Quick reference

Approved route
Subcutaneous (Vyleesi autoinjector). Intranasal is investigational and compounded.
Approved dose
1.75 mg SC, ≥45 min before sexual activity, per the package insert.
Approved frequency
At most 1 dose per 24 h and 8 doses per month.
Approved population
Premenopausal women with acquired, generalized HSDD.

PT-141 Protocol Formats

PT-141 Protocol Formats
ParameterValueSource
Dose1.75 mgVyleesi label / Mayo Clinic drug summary
RouteSubcutaneous (abdomen or thigh)Vyleesi label
Timing≥45 minutes before sexual activityVyleesi label
Maximum frequency1 dose per 24 hoursVyleesi label
Maximum monthly use8 doses per monthVyleesi label
Approved populationPremenopausal women with HSDDVyleesi label

Cycle Guidelines

Cycle Guidelines
ApproachPatternReference Source
FDA-approved on-demand (Vyleesi)1.75 mg subQ ≥45 min before activity, ≤1/24h, ≤8/monthVyleesi label (Mayo Clinic summary)
Phase 3 trial schedule1.75 mg subQ on demand for 24 weeksKingsberg 2019 (RECONNECT)
Long-term safety extension1.75 mg subQ on demand for an additional 52 weeksSimon 2019 (open-label extension)
Phase 2 intranasal (men with ED)7.5-20 mg intranasal in trial arms studiedDiamond 2006

PT-141 Reconstitution Guide

PT-141 Reconstitution Guide
BAC water addedFinal concentration0.10 mL draw deliversVolume to deliver 1.75 mg
1 mL10 mg/mL1.0 mg0.175 mL (~17.5 units on a U-100 syringe)
2 mL5 mg/mL0.5 mg0.35 mL (~35 units on a U-100 syringe)
2.5 mL4 mg/mL0.4 mg0.4375 mL (~43.75 units on a U-100 syringe)

PT-141 Timeline & What to Monitor

PT-141 Timeline & What to Monitor
Time after subQ doseWhat was reportedSource context
0-30 minutesPossible flushing; nausea may beginVyleesi label / RECONNECT trial reports
30-60 minutesPharmacodynamic effects beginPhase 3 trial protocol design
1-3 hoursReported peak effect windowTrial-reported timing
4-12 hoursEffects taper; nausea typically resolves within hoursVyleesi label

Storage and handling

The rules below apply to practically every lyophilized peptide in this reference. Where a compound has its own requirement, it appears in the table in the previous section.

  • Lyophilized powder, sealed: refrigerator, between 2 and 8 °C, protected from light. Many tolerate room temperature for short transport periods, but that is tolerance, not a recommendation.
  • After reconstitution: always refrigerated, between 2 and 8 °C. The stability window drops to days or a few weeks, depending on the compound.
  • Never freeze after reconstituting. The freeze–thaw cycle degrades the peptide.
  • Do not shake. Swirl the vial slowly. Shaking breaks the peptide chain.
  • Bacteriostatic water down the wall of the vial, in a slow stream, not squirted directly onto the powder.
  • Cloudy solution, with particles or a color change: discard. There is no recovery.

Lab tests and monitoring

This list is the one that appears recurrently in the source, with small variations by compound. It serves as a starting point for a conversation with a professional — not as a substitute for that conversation.

  • Before starting: complete blood count, comprehensive metabolic panel, lipid panel, fasting glucose and HbA1c, TSH and free T4, blood pressure and resting heart rate.
  • Depending on the compound: IGF-1 (GH axis), lipase and amylase (VIP and the incretin agonists), serum copper and ceruloplasmin (GHK-Cu and blends containing it), CRP.
  • Reassessment: most protocols review between week 4 and week 8, and then every 8–12 weeks.
  • Do not wait for the routine lab test in the face of abdominal pain, visual changes, a change in a mole, shortness of breath, asymmetric swelling or any new and persistent symptom. That is a reason to seek care, not a spreadsheet item.

Evidence limits

What these numbers are and what they are not. The values above were preserved exactly as they appear in the source, without reinterpretation. What the source describes as community practice is marked as such in the tables; what came from a published trial is too. A dose repeated by many people does not become a validated dose by repetition.

Data source: peptidedosingprotocols.com, accessed on September 3, 2026. Translation and organization in Portuguese are original work. No primary source (PubMed, trial registry, package insert) was checked in building this page — the check was against the secondary source, and that alone.

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